Protocol·Digestion·Beginner·Reviewed June 9, 2026
Gut Lining Repair Protocol.
A targeted stack to support the integrity of the intestinal mucosal barrier and soothe irritated gut lining. It combines mucosal-protective, anti-inflammatory, and microbiome-supporting agents to help restore healthy digestion.
The gut lining repair protocol in brief.
A quick summary. The full stack, with dose and timing for each supplement, is below.
The Gut Lining Repair Protocol is a beginner stack of 6 supplements aimed at digestion: L-Glutamine, Zinc Carnosine, Deglycyrrhizinated Licorice (DGL), Probiotics, Slippery Elm, and Inulin. 3 are core and the rest are optional add-ons, at roughly $40-70/mo. Each supplement below lists its dose, timing, role, and the evidence behind it.
What is in the gut lining repair protocol.
Dose, timing, role, and evidence tier for each supplement. Core items carry the protocol; optional ones are situational. Open any name for the full profile.
| Supplement | Dose | Timing | Role | Evidence |
|---|---|---|---|---|
| L-Glutamine | 5 g once or twice daily | On an empty stomach, between meals | Core | Moderate |
| Zinc Carnosine | 75 mg twice daily | With or after meals | Core | Moderate |
| Deglycyrrhizinated Licorice (DGL) | 380-760 mg chewed before meals | 20 minutes before meals | Optional | Emerging |
| Probiotics | 10-20 billion CFU daily | With food, morning | Core | Moderate |
| Slippery Elm | 400-500 mg up to three times daily | Between meals with water | Optional | Emerging |
| Inulin | 5 g daily, titrate up slowly | With a meal | Optional | Moderate |
Glutamine is the primary fuel source for enterocytes and supports tight-junction integrity, making it a foundational nutrient for repairing a compromised intestinal barrier.
Zinc carnosine stabilizes the gastric and intestinal mucosa, promotes epithelial migration and repair, and helps maintain gut wall integrity under inflammatory stress.
DGL increases mucus secretion and the protective mucosal layer of the upper GI tract while avoiding the blood-pressure effects of whole licorice, soothing irritated lining.
A healthy microbiome reinforces barrier function by stimulating mucin production and tight-junction proteins, helping crowd out pathogens that perpetuate gut irritation.
Slippery elm forms a demulcent mucilage that coats and soothes the gut lining, providing symptomatic relief while the barrier-repairing agents work.
As a prebiotic fiber, inulin feeds beneficial bacteria that produce short-chain fatty acids like butyrate, the main energy source for colonocytes and a key driver of barrier repair.
How the pieces combine.
The mechanistic rationale for stacking these together rather than taking them in isolation.
- L-Glutamine and zinc carnosine target enterocyte repair from complementary angles, fueling cell turnover while stabilizing the mucosal surface.
- Inulin feeds the probiotic strains it is paired with, and the resulting short-chain fatty acids reinforce the same tight-junction integrity that glutamine supports.
- DGL and slippery elm both add protective mucilage and mucus layers, buying symptom relief while the deeper repair agents act.
Cost and commitment.
A rough monthly cost and how involved the protocol is to run.
The evidence behind it.
Overview citations for this protocol. Each supplement's own profile carries its full source list.
- Camilleri M. Leaky gut: mechanisms, measurement and clinical implications in humans. Gut. 2019.
- Bischoff SC, Barbara G, Buurman W, et al. Intestinal permeability - a new target for disease prevention and therapy. BMC Gastroenterology. 2014.
Common questions.
Quick answers drawn from the stack above.
What is in the Gut Lining Repair Protocol?
The Gut Lining Repair Protocol combines 6 supplements for digestion: L-Glutamine, Zinc Carnosine, Deglycyrrhizinated Licorice (DGL), Probiotics, Slippery Elm, and Inulin. 3 are core; the rest are optional.
How much does the Gut Lining Repair Protocol cost?
NutriStack estimates the Gut Lining Repair Protocol at about $40-70/mo, depending on the forms and brands you choose and whether you run the optional add-ons.
Is the Gut Lining Repair Protocol backed by evidence?
Each supplement in the protocol carries its own evidence tier (0 rated strong here) and links to PubMed-cited sources. NutriStack does not rank or score brands and takes no manufacturer payments; this is an informational reference, not medical advice.
Build it in the app
Run the gut lining repair protocol in NutriStack.
Add the stack to NutriStack to track timing, screen it for interactions, and see a Stack Score that updates as you tune it.