NSTK · 01.2026Independent supplement reference
NutriStack
Edition 1.0Updated August 1, 2026

Supplement × Supplement·Evidence index

Probiotics and Vitamin D3: what the published research says

PubMed indexes 8 records that name Probiotics and Vitamin D3 together, either in the title or in an interaction, co-administration, or pharmacokinetic context. Each one is listed below with its study design and, where the abstract states one, the authors’ own conclusion, quoted and linked. This page reports that literature. It does not tell you whether to combine them.

Read this first

An evidence index, not guidance.

Every statement below is attributed to a named, dated, linked source.

This page lists published research relevant to Probiotics and Vitamin D3. It deliberately does not state whether the combination is safe, whether it interacts, in which direction, at what dose, or how far apart to take them.

The research

8 studies naming Probiotics and Vitamin D3.

Strongest relevance first: papers naming both substances in the title, then human trials and systematic reviews.

Preparation and characterization of Lactobacillus rhamnosus, Lactobacillus casei, glycyrrhizin, vitamin-D3, and citric acid-loaded mucoadhesive pectin microparticles for the management of dental caries.

BMC pharmacology & toxicology · 2025 · PMID 41310863

Animal study

“CONCLUSION: This study underscores the potential of probiotic-based mucoadhesive drug delivery systems in oral health management. It highlights their role in reducing reliance on conventional antibiotics, mitigating bacterial resistance, and improving patient compliance.”

Mishra et al. · From the abstract

Names both Probiotics and Vitamin D3 in its title.

Coadministration of doxorubicin with vitamin D3, Lactobacillus acidophilus, and Lactobacillus casei in the 4T1 mouse model of breast cancer: anticancer and enteroprotective effects.

Medical oncology (Northwood, London, England) · 2024 · PMID 38592504

Animal study

“The treated groups (except 6) had significantly higher expression of Bax/Caspase 3 genes and lower expression of Bcl-2 genes than the control group (P < 0.05, 0.01). Coadministration of Dox with probiotics and vitamin D3 showed promising results in reducing tumor size, protecting intestinal tissue and influencing gene expression, suggesting a strategy to enhance the effectiveness of breast cancer treatment while reducing side effects.”

Mirzadeh et al. · From the abstract

Names both Probiotics and Vitamin D3 in its title.

The Cooperation of Bifidobacterium longum and Active Vitamin D3 on Innate Immunity in Salmonella Colitis Mice via Vitamin D Receptor.

Microorganisms · 2021 · PMID 34576700

“It suggests the synergistic effects of Bifidobacterium longum and active vitamin D on the antibacterial and anti-inflammatory responses in Salmonella colitis. Therefore, our in vivo studies demonstrated that the combination of probiotic Bifidobacterium longum and active vitamin D3 has the synergistic effects on reducing the severity of Salmonella colitis via the suppression of inflammatory responses, and blocking the translocation of bacteria through the enhancement of…”

Huang et al. · From the abstract

Names both Probiotics and Vitamin D3 in its title.

The Health Effects of Vitamin D and Probiotic Co-Supplementation: A Systematic Review of Randomized Controlled Trials.

Nutrients · 2020 · PMID 33396898

Systematic ReviewHuman subjects

“Co-supplementation of vitamin D and probiotics generated greater health benefits than its comparators did. More studies in other diseases and various populations are needed to confirm these findings and to elucidate the optimal form, composition, and frequency of this co-supplementation.”

Abboud et al. · From the abstract

Names Probiotics and Vitamin D3 together in an interaction, co-administration, or pharmacokinetic context.

Limitations

What this evidence cannot tell you.

Search-based evidence indexes have real limits, and it is worth being explicit about them:

NutriStack

Track what you actually take.

NutriStack is a free iPhone app for organising a supplement routine: what you take, when, and what the published record says about it. Its exploratory interaction checker covers Probiotics, Vitamin D3, and several hundred other substances.

The checker is a research and organisation tool, not a clinical decision aid, and its per-pair assessments are excluded from search until they pass claim-level review.

NutriStack is an informational and organizational tool, not a medical service, and not a substitute for professional advice. Always consult a qualified healthcare professional before starting, stopping, or changing any supplement or medication.