NSTK · 01.2026Independent supplement reference
NutriStack
Edition 1.0Updated August 1, 2026

Supplement × Supplement·Evidence index

Fish Oil and Vitamin D3: what the published research says

PubMed indexes 8 records that name Fish Oil and Vitamin D3 together, either in the title or in an interaction, co-administration, or pharmacokinetic context. Each one is listed below with its study design and, where the abstract states one, the authors’ own conclusion, quoted and linked. This page reports that literature. It does not tell you whether to combine them.

Read this first

An evidence index, not guidance.

Every statement below is attributed to a named, dated, linked source.

This page lists published research relevant to Fish Oil and Vitamin D3. It deliberately does not state whether the combination is safe, whether it interacts, in which direction, at what dose, or how far apart to take them.

The research

8 studies naming Fish Oil and Vitamin D3.

Strongest relevance first: papers naming both substances in the title, then human trials and systematic reviews.

Effect of Vitamin D3, Omega-3 Fatty Acids, and Exercise on Serum Sclerostin Levels and Bone Turnover Markers.

The Journal of clinical endocrinology and metabolism · 2025 · PMID 39657964

Randomized Controlled TrialMulticenter Study1848 participants

“In this 3-year prevention trial among largely vitamin D replete adults age 70 and older, SHEP alone or in combination with omega-3s reduced serum sclerostin levels, while vitamin D3 and omega-3s alone did not affect serum sclerostin levels.”

Tsourdi et al. · Authors' conclusion

Names both Fish Oil and Vitamin D3 in its title.

Effects of vitamin D3, omega-3 fatty acids and a simple home exercise program on change in physical activity among generally healthy and active older adults: The 3-year DO-HEALTH trial.

The journal of nutrition, health & aging · 2025 · PMID 40054416

Randomized Controlled TrialMulticenter Study

“Among generally healthy, active, and largely vitamin D3 replete adults aged 70 years and older, vitamin D3, omega-3s and SHEP, individually and in combination had no benefits on self-reported PA and objectively measured physical function. The detrimental effect of vitamin D supplementation on PA change needs further examination.”

Hussein et al. · Authors' conclusion

Names both Fish Oil and Vitamin D3 in its title.

Vitamin D3 promotes fish oocyte development by directly regulating gonadal steroid hormone synthesis†.

Biology of reproduction · 2024 · PMID 38145497

Animal study

“Furthermore, the current study identified a vitamin D response element in the cyp19a1a promoter and demonstrated that 1.25(OH)2D3-vitamin D response directly activated cyp19a1a production through activating the vitamin D response element. In conclusion, this study shows that an appropriate concentration of vitamin D3 can promote zebrafish ovarian development and affect vitellogenin synthesis through the vdr/cyp19a1a/er/vtg gene axis.”

Yang et al. · From the abstract

Names both Fish Oil and Vitamin D3 in its title.

Vitamin D3 and omega-3 polyunsaturated fatty acids have beneficial effects on fracture union in an experimental rat model.

Joint diseases and related surgery · 2024 · PMID 38108173

Animal study

“The use of omega-3 PUFA together with vitamin D3 might have beneficial influences on fracture union. In the future, the combination of omega-3 PUFA and vitamin D3 might be used as an encouraging treatment choice that contributes to fracture healing.”

Kafadar et al. · Authors' conclusion

Names both Fish Oil and Vitamin D3 in its title.

Improving autism perinatal risk factors: A systematic review.

Medical hypotheses · 2019 · PMID 31088644

Systematic ReviewHuman subjects

“Studies of individual components for improving pregnancy outcomes and several uncontrolled preconception to infancy medical practices suggest that multiple interventions might improve the outcomes of pregnancies where there is risk for developing ASD.”

Cheng et al. · Authors' conclusion

Names Fish Oil and Vitamin D3 together in an interaction, co-administration, or pharmacokinetic context.

Effects of vitamin D3, omega-3s, and a simple strength training exercise program on bone health: the DO-HEALTH randomized controlled trial.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2024 · PMID 38613445

Randomized Controlled TrialMulticenter Study

“In contrast, participants receiving daily vitamin D supplements experienced a benefit at the hip. However, it should be noted that the effect across 3 years was very small.”

Kistler-Fischbacher et al. · From the abstract

Names Fish Oil and Vitamin D3 together in an interaction, co-administration, or pharmacokinetic context.

Effect of Daily Vitamin D Supplementation on Risk of Upper Respiratory Infection in Older Adults: A Randomized Controlled Trial.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2024 · PMID 38113446

Randomized Controlled TrialResearch Support, N.I.H., Extramural

“In older adults not selected for vitamin D deficiency, supplemental vitamin D did not lower URI risk overall. Whether effects differ in subgroups requires further study. Clinical Trials Registration. NCT01169259.”

Camargo et al. · Authors' conclusion

Names Fish Oil and Vitamin D3 together in an interaction, co-administration, or pharmacokinetic context.

Limitations

What this evidence cannot tell you.

Search-based evidence indexes have real limits, and it is worth being explicit about them:

NutriStack

Track what you actually take.

NutriStack is a free iPhone app for organising a supplement routine: what you take, when, and what the published record says about it. Its exploratory interaction checker covers Fish Oil, Vitamin D3, and several hundred other substances.

The checker is a research and organisation tool, not a clinical decision aid, and its per-pair assessments are excluded from search until they pass claim-level review.

NutriStack is an informational and organizational tool, not a medical service, and not a substitute for professional advice. Always consult a qualified healthcare professional before starting, stopping, or changing any supplement or medication.